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Environmental Applications Of Nucleic Acid Amplification Technology

RRP $906.99

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From the Preface
Antibody techniques have allowed us to study microorganisms in situ. However, until recently all methodology lacked the sensitivity necessary for environmental work where microorganisms are in most cases present at very low concentrations or where microbial ecosystems contain a myriad of different organisms. Gene probes have been used successfully for a variety of samples, but this method was still not sensitive enough. The next logical step was the application of the recently developed DNA amplification technique known as the polymerase chain reaction, or PCR. Since then, many laboratories around the world have adopted PCR for environmental work.
Samples obtained from soils, water and air are enormously complex because they are unknown mixtures of DNA and other compounds. Thus, procedures for target DNA amplification from the environment require special attention. The PCR has allowed us to go beyond the need for culturing prior to analysis of microbial communities. It has been shown that even microorganisms that can be routinely grown in the laboratory undergo some physiological changes when exposed to the environment. One of these changes (first observed by R. Colwell and colleagues) is known as the viable-but-non-culturable state, and seems to be a common occurrence. Thus, the use of culture techniques paint only part of the picture in terms of microbial behavior under environmental conditions. The ability to amplify nucleic acids by the PCR has brought about a myriad of very ingenious modifications to the technique that can then be used to study complex ecosystems. The manner in which the PCR can be modified is only limited by the need and/or the imagination of the researcher.
The first manual dedicated specifically to the analysis (by PCR) of environmental samples, Environmental Applications of Nucleic Acid Amplification Techniques presents state of the art methodology for the detection of microorganisms in soil, water, air samples, as well as the amplification of nucleic acids from fossil samples. The manual gives step-by-step procedures for the analysis of these samples. Although several publications have addressed the use of Polymerase Chain Reaction technique, very few of them have been directed toward the application of this technique to environmental samples. This book fills this gap in the literature.

The Economic And Environmental Impacts Of Agbiotech

RRP $24.99

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After almost fifteen years in the laboratory and in the test plots, bioengineered crops arrived to the market in the mid-1990s. Adoption was rapid and wide- spread. In 1996, less than 4 million acres in six countries were planted with bioengineered crops. By 2001, worldwide adoption had expanded to more than 115 million acres. Important questions quickly followed. What were the factors driving the widespread adoption and rapid diffusion of these first-generation agrobiotech- nologies? What were their economic and environmental impacts? How were such impacts distributed among large and small producers, innovators and adopters, developed and developing countries, exporters and importers, domestic and foreign consumers? How were such impacts and their distribu- tion affected by market structure and government policies? A growing body of literature has provided valuable answers to some of these questions. However, an assessment that accounts for the full range of differences in geography, weather, pests, farm structures, and institutions had not been completed. It brings together leading This book provides just such an assessment. authors from around the world who have analyzed the production, environ- mental, and economic impacts of first-generation crop biotechnologies. By pooling experiences across various countries, time periods, crops, and traits, this global panel is able to synthesize a complete picture of the impacts of first-generation crop biotechnologies.

Ventilation For Control Of The Work Environment, Second Edition

RRP $436.99

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The second edition of Ventilation Control of the Work Environment incorporates changes in the field of industrial hygiene since the first edition was published in 1982. Integrating feedback from students and professionals, the new edition includes problems sets for each chapter and updated information on the modeling of exhaust ventilation systems, and thus assures the continuation of the book's role as the primary industry textbook.<br> This revised text includes a large amount of material on HVAC systems, and has been updated to reflect the changes in the Ventilation Manual published by ACGIH. It uses both English and metric units, and each chapter concludes with a problem set.

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